Possible Interaction: Leukotriene C4 and Serine

“In contrast, verlukast (20-200 nM) failed to antagonize contractions of GPT induced by leukotriene C4 in the presence of 45 mM L-serine borate.”

“ L-Serine borate complex, 45 mM, … the degradation of LTC4 to LTD4 by the enzyme gamma-glutamyl transpeptidase, in paired airway segments (adjacent segments from the same branch), produced a …-fold) of shift to the right of the dose-response curve and reduction of the maximum response to LTC4.”

“Inhibition of LTC4 bioconversion to LTD4 by pretreatment with L-serine (100 mumol/L) prevented the LTC4-induced increase in leukocyte rolling flux and the LTC4-induced reduction in leukocyte rolling velocity.”

“In separate experiments, responses to LTC4 were also reduced by approximately 50% if LTC4 conversion to LTD4 and LTE4 was first prevented by L-serine borate (45 mM) in combination with L-cysteine (10 mM).”

“ L-Serine borate (45 mM), an inhibitor of gamma-glutamyl …, shifted the dose-response curve of LTC4 to the left by 161-fold, and L-cysteine (6 mM), an inhibitor of aminopeptidase, shifted … dose-response curves of LTC4 and LTD4 to the left by 67- and 23-fold, respectively.”

“ LTC4 was significantly more potent in the presence of L-serine borate , an inhibitor of gamma-glutamyl transpeptidase, than in its absence, raising the possibility of significant bioconversion of LTC4 by the bullfrog heart.”

“ L-Serine borate (45 mM) enhanced the contractile activity of LTC4 without altering the response to LTD4.”

“The bioconversion of [3H]LTC4 was blocked by L-serine borate in a concentration-related manner (IC50 = 3.4 +/- 0.5 mM, mean +/- S.E.M., n = 3) and the formation of LTE4 was blocked by L-cysteine (10 mM).”

“ L-Serine borate (45 mM) increased selectively the contractile activity of LTC4.”

“ L-Serine borate , an inhibitor of gamma-glutamyl transpeptidase prevents the conversion of LTC4 to LTD4.”