Possible Interaction: Leukotriene B4 and Calcium Supplement

“ LTB4 functionally mobilized intracellular calcium and inhibited forskolin-stimulated cAMP production in 293 cells.”

“In stably transfected Chinese hamster ovary cells, LTB4 inhibited forskolin-activated cAMP production and induced an increase of intracellular calcium , suggesting that this receptor is coupled to Gi- and Go-like proteins.”

“In CHO cells stably expressing this receptor, LTB4 induced increases in intracellular calcium , D-myo-inositol-1,4,5-triphosphate (InsP3) accumulation, and inhibition of adenylyl cyclase.”

“Both ionophore- and Ig-induced LTC4 and LTB4 release were completely inhibited by removal of calcium from the media and by preincubation of cells with nordihydroguaiaretic acid.”

“Addition of f-Met-Leu-Phe (10(-9) M), C5a (3 x 10(-9) M) or leukotriene B4 (6 x 10(-8) M) to the neutrophils induces a rapid increase in the intracellular concentration of free calcium that reaches a maximum value 15 seconds following stimulation.”

“RESULTS We demonstrate that oxCL but not its non-oxidized counterpart CL induces biosynthesis of LT 4 an d increases intracellular concentrations of cal cium in monocytes/macrophages and neutrophils.”

“In addition, LTB4 concentrations correlated significantly with the calcium content of the aortic valves.”

“Stimulation of neutrophils with formyl-methionine-leucine-phenylalanine, IL-8, and LTB4 inhibited subsequent mobilization of calcium by the supernatant.”

“ Leukotriene B4 causes a rise in calcium and a decrease in light scatter.”

“The synthetic chemotactic peptide, f-Met-Leu-Phe, also elicits formation of LTC4 and LTB4 in a dose-dependent manner in the presence of exogenously added calcium.”