Possible Interaction: Leukotriene B4 and Arachidonate

“Multiple additions of LTB4 , which would be expected to more closely resemble the situation in vivo where the cell may come into contact with an environment where LTB4 is in near constant supply, yielded a more sustained release of arachidonate.”

“Since the affinity for arachidonate of the omega-monooxygenase was quite low and the presence of LTB4 suppressed the omega-hydroxylation of arachidonate , we conclude that the known LTB4 omega-monooxygenase is responsible for the formation of omega-hydroxy-arachidonate.”

“Subsequent incubation with arachidonate resulted in an up to tenfold-stimulated formation of 5-hydroxyeicosatetraenoic acid, leukotriene B4 , leukotriene B4 isomers and omega-hydroxyleukotriene B4.”