Possible Interaction: Leucine and Genistein

“The lignans and genistein were strongly suppressive against incorporations of [3H]thymidine, [3H]uridine, and [3H]leucine into HL-60 cells.”

“CONCLUSION The individual administration of le cine an d gen istein ha d less anti dopaminergic activity when compared with their combined administration.”

“Pretreatment with leucine and genistein significantly (p<0.01, 55.5 +/- 5.898 minutes) potentiated the haloperidol induced catalepsy compared with the haloperidol treated group.”

“1) GST (10, 50, 100 micromol/L) reduced the voltage-activated peak amplitude of I(Ca, L ) in a concentration-dependent manner.”

“3) GST markedly shifted the steady-state inactivation curve of I(Ca, L ) to the left, and accelerated the voltage-dependent steady-state inactivation of I(Ca,L).”

“4) GST markedly shifted the curve of time-dependent recovery of I(Ca, L ) from the steady-state inactivation to the right, and slowed down the recovery of I(Ca,L) from inactivation (n=7, P<0.01). (”

“From the results obtained it is concluded that genistein inhibits I(Ca, L ) and acts on the inactivated state of L-type calcium channel.”

“Exposure to GEN also increased the number of splenic B cells ( L ), macrophages (L and M), T cells (H), helper T cells (L and H), and cytotoxic T cells (M and H).”

“Furthermore, exposure to GEN increased the number of splenic B cells (H), T cells ( L , M, and H), and T-cell subsets (L, M, and H).”

“In F(1) females, exposure to GEN produced a decrease in terminal body weight (H), with an increase in the relative weight of spleen ( L , M, and H).”

“In F(1) males, exposure to GEN gestationally, lactationally, and through feed from Postnatal Days 22 to 64 (total 78 days) produced an increase in the relative weights (% body) of spleen (L and H) and thymus ( L).”

“At the highest concentration of isoflavone tested (200 microM), ICa L was inhibited by 66 +/- 7% ( Gst ), 22 +/- 2% (daidzein), and 1 +/- 3% (genistin).”

“ Gst inhibited ICa, L (IC50 = 47 microM) without affecting its time course or shifting the ICa, L-voltage relationship.”

“ Gen (50 microM) elicited biphasic changes in ICa, L : an initial inhibition (-55 +/- 4%; phase 1) followed by a secondary stimulation (34 +/- 9%; phase 2) of ICa,L. Withdrawal of Gen elicited a further potentiation of ICa,L (152 +/- 19%; phase 3) above control (n = 46).”

“At the highest concentration of isoflavone tested (200 μM), I Ca L was inhibited by 66 ± 7% ( Gst ), 22 ± 2% (daidzein), and 1 ± 3% (genistin).”

“ Gen (50 μM) elicited biphasic changes in I Ca, L : an initial inhibition (-55 ± 4%; phase 1) followed by a secondary stimulation (34 ± 9%; phase 2) of I Ca,L. Withdrawal of Gen elicited a further potentiation of I Ca,L (152 ± 19%; phase 3) above control ( n = 46).”

“When cells were dialyzed using a ruptured patch recording method, Gen elicited only inhibition of I Ca, L ; phases 2 and 3 were abolished.”