Possible Interaction: Isoflurophate and Trypsin

“The burst-stimulating effect of trypsin is enzymatic since it is completely prevented by DFP , a specific inhibitor of trypsin action.”

“Thus pand y-chymotrypsins behave toward DFP in quite the same manner as the c11 … (5) (which may not be astonishing in view of their general similarity) ; however, DFP also … with trypsin to produce a crystallizable, inhibited trypsin whose equivalent weight … respect to phosphorus is about 20,000 (5).”

“ Diisopropyl fluorophosphate-inactivation of trypsin inhibited PMNL accumulation.”

“Neither alveolar type II cells nor mesothelial cells showed increased labeling when trypsin inactivated by diisopropyl fluorophosphate was used.”

“Abstract The high-resolution structure of bovine trypsin inhibited with DFP † was determined by Stroud et al. (1971 and R. M. Stroud, L. M. Kay, A. Cooper & R. E. Dickerson, Abstr.”

“The concentration of DFP employed was sufficient to have inhibited the esterolytic activity of proteases such as trypsin or chymotrypsin, if present.”

“ Trypsin , alpha-chymotrypsin, and subtilisin were inactivated with diisopropyl fluorophosphate , and the distance of the phosphorus from Gd(III) in place of Ca(II) was determined from the lanthanide-induced relaxation on the 31P resonance.”

“ Trypsin inactivated by diisopropylphosphorofluoridate fails to occupy inhibiting sites on α-1-antitrypsin.”

“Such studies have shown that the reaction of trypsin with diisopropyl fluorophosphate (3) or diethyl p-nitrophenyl phosphate (DENP) (4) is accompanied by a loss in the proteolytic, esterase, and amidase activities of this enzyme.”

“ Trypsin and chymotrypsin are both inhibited by small amounts of DFP (1).”