Possible Interaction: Interferons and Genistein

“This was further supported by the observation that genistein (protein tyrosine kinase inhibitor) inhibits IFN –Á production for Th1 and IL–4 for Th2 in a dose–dependent manner.”

“The tyrosine protein kinase inhibitor, genistein , inhibited the production of inositol phosphates and the elevation of [Ca2+]i induced by IFN gamma, but had no effect on ATP, suggesting that tyrosine protein kinase is involved in the signaling transduction of IFN gamma.”

“ Genistein also markedly attenuated the release of cytokines, including interleukin (IL)-2, IL-4, IL-5, interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha.”

“The tyrosine kinase inhibitor genistein inhibited NO production induced by IFN‐γ , suggesting that the signal transduction pathway leading to NOS induction in rat PMN involves phosphorylation by tyrosine kinase.”

“The inhibitory effect of I K,ATP by interferon‐α was blocked by genistein and herbimycin A, tyrosine kinase inhibitors, but was not affected by N‐(2‐metylpiperazyl)‐5‐isoquinolinesulfoamide (H‐7), an inhibitor of protein kinase C and cAMP‐dependent protein kinase.”

“ Genistein potently inhibited the productive interaction of macrophages with LPS but had only a minor effect on the action of interferon‐ γ.”

“ Genistein completely reversed the inhibition of thymidine kinase exerted by interferon , while quercetin had only a slight effect.”

“Treatment of HL-60 cells with the tyrosine kinase inhibitors, genistein and herbimycin A, inhibited both IFN gamma-stimulated tyrosine phosphorylation and IFN gamma-induced Fc receptor expression.”