Possible Interaction: Interferon Gamma-1B and Vitamin D

“ Vitamin D has the ability to suppress inflammatory cytokines such as TNF, IL-1, IFN-gamma and IL-2; while it increases the generation of anti-inflammatory cytokines IL-4 and IL-10.”

“Our results indicated that cotreating the RB-defective S4 cells with IFN-gamma and Vitamin D3 decreased the number of cells containing subdiploid DNA compared to cells treated with IFN-gamma alone, suggesting that Vitamin D3 reduced IFN-gamma-mediated apoptosis.”

“S4 cells cotreated with Vitamin D3 and IFN-gamma also had decreased cell detachment, further indicating that Vitamin D3 decreased IFN-gamma induced apoptosis.”

“Sequential treatment of these cells with IFN-gamma and VD3 (each 72 hr), or vice versa, showed significant suppression of HLA-DR antigen expression.”

“The fluorescens intensity of class I antigen was increased after IFN-gamma treatment, however, this effect is inhibited when the cells were treated with combination of IFN-gamma and VD3.”

“When these cells were cultured with both IFN-gamma and VD3 at the same time, the expression of HLA-DR antigen was significantly decreased.”

“The presence of vitamin D3 reduced IFN-gamma titers when PHA and IL-2 were used to induce IFN, but not when ionomycin was used as the inducer.”

“Pretreatment with vitamin D3 followed by IFN-gamma stimulation resulted in a 147% increase in C2 mRNA content compared with IFN-gamma stimulation alone.”

“ Vitamin D3 pretreatment followed by IFN-gamma stimulation resulted in earlier and greater production of C2.”