Possible Interaction: Interferon-Alpha and Methionine

“It indicated that the inhibitory effect of M on IFN-β activation is mainly through MAVS.”

“We found M overexpression significantly inhibited SeV-mediated IFN-β activation in a dose-dependent manner (Fig. 1i).”

“To confirm whether M inhibits viral RNA-induced IFN signaling, we treated the M-transfected cells with intracellular poly(I:C) and found that M reduced IFNβ signaling activation (Fig. 1b).”

“We then found that M decreased the IFNβ luciferase reporter activity induced by the active mutant of RIG-I [RIG-I (2CARD)] but not the downstream signaling proteins such as mitochondrial antiviral signaling protein, TBK1, or the active form of IRF3 [IRF3(5D)] (Fig. 1c).”

“Furthermore, MET markedly upregulated the levels of IFN-γ production and downregulated those of IL-10 in isolated MOs, while inducing a slight opposing up-regulation of IL-10 production in co-cultures.”

“Treatment of co-cultures with MET resulted in increased release of antitumor cytokine IFN-γ and ifCa2+, and increased cell necrosis during breast cancer cells-MOs crosstalk.”

“Treating Huh7 cells with IFNα plus oncostatin M increased IL-7 and IL-15Rα mRNA more intensely than either cytokine alone.”

“Furthermore, the researchers found that, in line with previous studies, the combination of IFN-α and oncostatin M upregulated IL-7 and IL-15Rα.”

“RESULTS Mean levels of IN alpha an d TNF alpha in patients with M ( 7 5+/-47 and 304+/-102 rg/ml respectively) were significantly higher than in patients with MCS and healthy donors (31+/-14 and 83+/-39; 38+/-18 95+/-58 rg/ml, respectively, p<0.05).”

“Cytokine ELISA analysis revealed that co-injection of M could enhance the levels of IFN-γ , IL-2 release induced by N antigen.”