Possible Interaction: Hydroxyproline and Vitamin C

“In particular, collagen synthesis seems to be required for tube formation because cis-hydroxyproline inhibited tube formation, whereas either the presence of ascorbic acid or the addition of exogenous collagen IV to the Matrigel increased tube formation.”

“The latter caused a statistically significant rise and normalization respectively of plasma ascorbic acid and simultaneously a statistically significant increase of the hydroxyproline and proline in periodontal tissue (p less than 0.01) but not before the plasma vitamin C level was above 0.9 mg/dl.”

“The hydroxyproline levels were significantly high in ascorbic acid plus omega-3 fatty acid combination group than the other groups (p < 0.05).”

“Pretreatment with ascorbic acid augmented the synthesis of collagen significantly as revealed by an increase in hydroxyproline content.”

“However, ascorbic acid produced a dose-dependent decrease in the hydroxyproline content, with a maximal 76.8% decrease in bones without ascorbic acid compared with the control bones with 100 microg/ml ascorbic acid.”

“An inhibitor of collagen synthesis, cis-hydroxyproline (cis-OH-Pro), suppressed the ascorbic acid-induced polarity reversal.”

“The stimulatory effect of ascorbic acid on collagen production is detectable by a 20-27% increase in the concentration of hydroxyproline in the culture medium from the two glioma cell lines.”

“Actions of ascorbic acid on osteoblast marker gene expression are mediated by increases in collagen synthesis and/or accumulation because (1) … ascorbic acid stimulation of collagen accumulation and alkaline phosphatase activity, and (2) the specific collagen synthesis inhibitors, 3,4‐dehydroproline and cis‐4‐hydroxyproline, … ascorbic acid‐dependent … expression.”

“ Ascorbic acid also stimulated the synthesis of collagen and increased the hydroxyproline content of MC3T3-E1 cell cultures markedly.”

“Addition of ascorbic acid to the culture medium caused in concentrations from 2.5 micrograms/ml an increase of the hydroxyproline content and of the size and in concentrations from 20 or 40 micrograms/ml, resp.,”