Possible Interaction: Hydrogen Peroxide and Vitamin K

“The results showed that the addition of menadione can induce oxidative stress on myoblasts by superoxide and hydrogen peroxide production, which can be eradicated by superoxide dismutase (SOD) and catalase, respectively, in a dose-dependent mode.”

“Preincubation of Hep G2 cells with low, nontoxic concentrations of menadione increased the viability of the cells against toxic doses of menadione or H2O2.”

“The production of H2O2 by intact cells is promoted in the presence of menadione and is proportional to the density of viable cells.”

“The overall in vitro result showed that combination of trans‐chalcone, vitamin K1 and sorafenib (10, 5 and 5 μM concentration, respectively) enhanced the resistance against oxidative stress generated by H2O2.”

“These data suggest that menadione inhibits caspase activity by the generation of hydrogen peroxide through redox cycling and that caspase inactivation by this mechanism may prevent cell death by apoptosis in this oxidative-stress model.”

“Hydrogen peroxide-treatment of T-cells resulted in the formation of mitochondrial permeability transition pores, a rapid decrease of the mitochondrial transmembrane potential delta … Cytochrome C. Inhibition of the mitochondrial permeability transition by bongkrekic acid (BA), or interference with the mitochondrial electron transport system by rotenone or menadione … H2O2.”

“Significant tail moment increase, which is a marker of DNA strand breaks in the Comet assay, and intracellular nucleotide depletion occurred simultaneously in MeT-5A and BEAS 2B cells during the first 30-60 min of exposure to H2O2 and menadione.”

“L and C were also less susceptible to killing by H2O2 than was B311, and when treated with menadione , they each produced less H2O2 than did B311.”

“In addition, vitamin K2 could attenuate reactive oxygen species (ROS) level after exposure of cells to H2O2 for 24 h and Aβ (1-42) for 48 h. Cell apoptosis significantly increased following application of Aβ (1-42) (25 μM) and H2O2 (150 µM) compared to control.”

“ Vitamin K2 increased the amount of glutathione after exposure of cells to H2O2 for 24 h and Aβ (1-42) for 48 h. Western blot analysis of PC12 cells showed that 25 μM Aβ (1-42) and 150 µM H2O2 treatment could increase Bax, PARP cleavage, Phospho-p38 MAPK.”

“Subsequently, it was shown that 0.1 μM VK2 , at blastocyst stage, reduces H2O2 production, increase the expression of mitochondrial related gene and improved embryos quality.”