Possible Interaction: Hydrogen Peroxide and NADH

“Generation of the free radical O2•– and H2O2 by eGFP in the presence of NADH affects the gene expression of cells.”

“We showed that H2O2 initiates and maintains NADH oxidation and O2-uptake but is also necessary for the formation of superoxide anion and hydroxyl radicals.”

“Furthermore, an inhibitory effect of NADH on metHb formation was observed with additions of oxidants such as H2O2 , NO, and NaNO2.”

“ H2O2 (0.2–5 mM) moderately depolarized mitochondria, halted their intracellular trafficking in a Ca2+- and cAMP-independent manner, and directly oxidized cellular nicotinamide adenine dinucleotide ( NADH ) and flavin adenine dinucleotide (FADH2).”

“Recently it has been shown that NADH can be considered an important antioxidant as it reacts with peroxyl and alkoxyl radicals under in vitro conditions.”

“We have previously demonstrated that when cardiac mitochondria were challenged with H2O2, NADH production and oxidative phosphorylation declined.”

“They inhibited NADH production by acting specifically on aconitase for and alpha-ketoglutarate dehydrogenase for H2O2 and HO•. Our results show that , H2O2 and HO• act on different mitochondrial targets to alter ATP synthesis, mostly through inhibition of NADH production.”

“Addition of NADH or NADPH increased H2O2 production by a factor of 3 and 5, respectively.”

“Mutation of an NADH peroxidase-encoding gene resulted in the enhanced accumulation of hydrogen peroxide.”

“Alterations in NADH levels induced by H2O2 closely paralleled changes in the rate of state 3 respiration.”