Possible Interaction: Hydrogen Peroxide and NAD

“Intracellular NAD levels fell by 80% within 20 min of exposure of cells to H2O2.”

“Additionally, H2O2 treatment significantly increased the level of nicotinamide adenine dinucleotide phosphate (NADP+) and reduced the levels of ATP and NAD+.”

“In the present study, we showed that and 0.1 mM nicotinamide adenine dinucleotide (NAD+) administration significantly blocked RPE cell death induced by 300 μM H2O2.”

“ H2O2 induced lowering of NAD caused modest lowering of ATP but marked additional ATP-lowering, independent of PARP and NAD, was also demonstrated.”

“The results showed that H2O2 exposure caused oxidative damage in brain evidenced by decreased glutathione (GSH), total antioxidant capacity (T-AOC) and nicotinamide adenine dinucleotide (NAD+) levels, and increased formation of malondialdehyde (MDA) and 8-hydroxy-2'-deoxyguanosine (8-OHdG).”

“ NAD+ inhibits both Complex I-mediated and ammonium-stimulated H2O2 production.”

“Just after H2O2 exposure, ATP and NAD+ decreased by ≈50%, PCr decreased by 75%, and the ADP/ATP ratio approximately doubled.”

“Caffeine, ryanodine, cyclic ADP-ribose (endogenous ryanodine receptor agonist) and beta-NAD+ (precursor of cyclic ADP-ribose) suppressed partially the stimulatory effect of H2O2.”

“Cultured murine glial cells showed a decrease in intracellular NAD levels of >40% after treatment with H2O2 (100 µM).”

“Exposure to an oxidative stress by the addition of H2O2 resulted in a greater consumption of ATP and NAD in the transfected cells than in the wild-type cells.”