Possible Interaction: Hydrogen Peroxide and Green Tea Extract

“ Green tea polyphenols can also be potent pro-oxidants, both in vitro and in vivo, leading to the formation of hydrogen peroxide , the hydroxyl radical, and superoxide anion.”

“ Tea extracts showed potential scavenging of H2O2 and NO, appreciable extent of total antioxidant capacity and effective anti-hemolytic action.”

“In the present study, we found that green tea polyphenol significantly inhibited IL-8 production induced by hydrogen peroxide (H(2)O(2)) in human lung alveolar epithelial cells (A549 line).”

“… green tea polyphenols includes … peroxyl radicals with the activity sequence EC>EGCG>ECG>EGC>GA for the AAPH initiated peroxidation, and reducing the alpha-tocopheroxyl radical to … sequence of ECG>EC>EGCG>EGC>GA and ECG>EGCG>GA>EC>EGC for the AAPH-initiated and BP-photosensitized peroxidations respectively.”

“It appeared … pretreatment with GTP inhibited both cigarette smoke- and H2O2-induced DNA …; i.e., following exposure to cigarette smoke or H2O2 , the fraction of DNA passing through a microfilter increased significantly in cells not subjected to GTP, but this effect was prevented or inhibited in GTP-treated cells.”

“Pretreatment with GTP also reduced the overall toxicity of H2O2 as determined by cell growth after exposure.”

“We conclude that the green tea extract in alcohol efficiently reduced the amounts of O2−, H2O2 and HOCl primarily due to the catechin content, and not caffeine.”

“Addition of GTE (500 ppm) and TOC (500 ppm) increased the formation of hydroperoxides and certain VOCs.”

“ GTE also decreased the intracellular ROS levels of EPCs induced by H2O2 at doses of 50, 100, 200 µM for about 84.24, 92.27, and 93.72% compared to controls, respectively.”

“Results: GTE ameliorated the cell viability of EPCs induced by H2O2 at doses of 50, 100, 200 µM for about 25.47, 22.52, and 11.96% higher than controls, respectively.”

“Furthermore, GTE supplementation in combination with H2O2 leads to a higher gene expression of osteocalcin and collagen1α1 during osteoblasts differentiation.”

“One-hour exposure of … 1 mg/ml GTP significantly increased the … (2 mM H2O2 , 4 mM tert-butylhydroperoxide), low pH solution (pH 4.0) containing various organic acids (60 mM citric, acetic, … acid) and other stress conditions (47 °C, 25 % NaCl, 12 % ethanol and 150 μg/ml crystal violet).”