Possible Interaction: Hydrocortisone and Leucine

“Salivary free cortisol is a non-invasive and practical alternative; however, when cortisol concentrations exceed 500 nmol · L there is a lack of agreement between salivary (free) and venous (bound) cortisol.”

“ Hydrocortisone treatment also resulted in increased incorporation of leucine into protein and decreased breakdown of pre-labeled protein in both confluent and logarithmically growing cultures.”

“ Hydrocortisone supplementation resulted in enhanced [3H]leucine uptake by the cells, inhibited in the presence of anti-rat albumin serum.”

“In non mitogen stimulated thymocytes, cortisol (10−6 M) inhibits leucine incorporation into protein by 30%, whereas in Con A stimulated cells, cortisol inhibits leucine incorporation by 50%.”

“The inhibitory effect of cortisol on leucine incorporation is dose and time dependent in both control and Con A treated cells.”

“ Cortisol inhibited the rate of [3H]leucine incorporation into protein during the initial 4h of incubation, but it had no effect at subsequent times.”

“In intact sensitive cells, free fatty acid (FFA) decreased the uptake of thymidine but did not mimic the other inhibitory effects of cortisol on uptake of deoxyglucose, leucine , or uridine.”

“ Hydrocortisone stimulated the catabolism of prelabeled fatty acyls in mouse L fibroblasts supplemented with exogenous fatty acid.”

“ Cortisol in vitro, at concentrations down to 10−7M, significantly decreases net glucose uptake by such cell preparations during the first hour of incubation, and at 10−6M (the only concentration tested) decreases the incorporation of leucine into protein during the second hour.”

“ Hydrocortisone inhibited the incorporation of glycine, leucine , phenylalanine and valine; incorporation of either methionine or serine was not affected.”