Possible Interaction: Histidine and Hydrogen Peroxide

“Micromolar concentrations of l‐histidine increase the cytotoxicity of hydrogen peroxide in a number of cell lines including CHO(hamster), EAHY, McCoy's, U937 and CCRF‐CEM (human), Vero (monkey) and SC‐1 (mouse).”

“These results strongly support the hypothesis that the mechanism whereby l‐histidine increases the toxicity elicited by H2O2 involves the formation of DNA DSBs and are consistent with the possibility that the amino acid might participate in the regulation of the physio‐pathological response to oxidative stress in mammals.”

“In addition, L‑histidine increased the generation of intracellular hydroxyl radicals in the presence of H2O2 in E. coli cells.”

“ L‑histidine increased the genomic fragmentation of E. coli MG1655 following exposure to H2O2.”

“Reverse transcription‑quantitative polymerase chain reaction analysis demonstrated that the expression of recA in E. coli MG1655 increased ~7‑fold following treatment with 10 mM hydrogen peroxide (H2O2) plus 1% L‑histidine , compared with that following exposure to H2O2 alone.”

“The combination of L‑histidine and H2O2 may be a useful strategy to selectively increase the microbicidal activity of oxidative agents against Gram‑negative bacteria.”

“The … mM H2O2 …% L‑histidine significantly reduced the number of viable cells of extended‑spectrum‑β‑lactamase‑producing E. coli and multidrug‑resistant Pseudomonas aeruginosa, and this … effective than 100 mM H2O2 alone, but this effect was not observed in methicillin‑resistant Staphylococcus aureus or vancomycin‑resistant Enterococcus faecium.”

“These results indicate that histidine inhibited the hydrogen peroxide‐ and TNF‐α‐induced IL‐8 secretion at the transcriptional level in intestinal epithelial cells, suggesting that histidine has the potential to attenuate intestinal inflammation.”

“We found that histidine , one of the conditionally essential amino acids, significantly inhibited both hydrogen peroxide‐ and TNF‐α‐induced IL‐8 secretion and mRNA expression in Caco‐2 cells and HT‐29 cells.”

“ L-Histidine (L-His) enhances the clastogenic effects of hydrogen peroxide (H(2)O(2)).”

“The formation of the adduct of L-His with H2O2 brings about the stabilization or reduces the reactivity of H2O2 and, as a result, the induction of SSB is prevented to some extent in cell-free DNA systems.”

“ L-Histidine , at a concentration of 1 mM, was found to potentiate both cell killing and inhibition of PHA-stimulated cell division brought about by hydrogen peroxide or xanthine oxidase plus hypoxanthine.”

“The formation of 8-OH-dG by H2O2 was increased about 3-folds in the presence of 78 microM L-histidine (L-His).”

“The result suggests that oxidative DNA damage induced by H2O2 in vitro might be enhanced by L-His.”

“Similar protective effects of the chelators and nitroxides were observed when L-histidine , which enhances intracellular injury, was added to H2O2.”

“ Histidine enhances the cytotoxic and clastogenic effects of hydrogen peroxide.”

“ Hydrogen peroxide is a potent inducer of DNA SSBs, and histidine modulates the formation of these lesions.”

“This effect has been extensively characterized with the use of purified DNA, and the results obtained have demonstrated that, upon exposure to low or high concentrations of H2O2, histidine reduces or enhances the formation of DNA SSBs, respectively.”

“ Histidine effectively quenched hydroxyl radicals and H2O2 , but not superoxide anions, in in vitro and in vivo conditions.”