Possible Interaction: Histidine and Aspartate

“Replacing aspartate in the S3‐S4 loop with histidine (D145H) enhanced both the slowing of activation kinetics and the shift in the voltage–conductance curve, such that Zn2+ inhibition closely resembled that of the human channel.”

“Mutation of Asp69 to histidine severely impairs NMO-IgG binding for 85.7% of the NMO patient sera analyzed here.”

“Although lysine, a conservative substitution in BMPRIA and BMPRIB, can be readily accommodated, histidine at residue 206 (like in fibrodysplasia ossificans progressiva) would participate in a salt bridge with the aspartate only at decreased intracellular pH and with extensive structural rearrangement.”

“As a possible functional role for the neutral aspartate in the active site, we propose that in the substrate-bound form, the neutral aspartate residue helps to keep the pKa of the histidine sufficiently low, in the active neutral form.”

“ L-Histidine also enhanced the excitatory action of L-glutamate, D- and L-aspartate , L-homocysteate and especially that of quisqualate, whereas the actions of acetylcholine, kainate, N-methyl-D-aspartate and D-homocysteate were more commonly reduced.”

“The coupling of an aspartate residue with an active site histidine plays a pivotal role in enzyme catalysis.”

“Substitutions of Thr-383 histidine in most PGHS-1) with histidine or aspartate decreased cyclooxygenase activation efficiency by about 40%, with little effect on cyclooxygenase specific activity or self-inactivation.”