Possible Interaction: Heparin and Trypsin

“Under certain conditions heparin protected the peptide from proteolysis by trypsin.”

“The affinity of normal EC-SOD (n-EC-SOD) for heparin decreased by the treatment with trypsin , accompanied by a reduction in the molecular mass.”

“The protection requires bioactive bFGF because inactivated bFGF is rapidly degraded by trypsin or chymotrypsin in the presence of heparin.”

“ Heparin inhibits proteolytic digestion of heparin-binding growth factor-I (HBGF-I) by trypsin , plasmin and other proteases.”

“ Heparin acted … of trypsin (Ki = 1.2 X 10(-6) M), plasmin (Ki = 4.9 X 10(-6) M) and urokinase (Ki = 1.0 X 10(-7) …-Val-Arg-p-nitroanilide (S-2160), H-D-Val-Leu-Lys-p-nitroanilide (S-2251) and plasminogen, respectively, were used as substrates.”

“It was found that the effect of heparin on the amidase activity of urokinase (E C 3.4.21.31), plasmin (E C 3.4.21.7) and trypsin (E C 3.4.21.4) depended on the substrate used.”

“In the presence of heparin , inhibitory effects of ulinastatin on trypsin were enhanced, whereas its effects on chymotrypsin and elastase were attenuated.”

“ Heparin also decreases the ka for the elastase/eglin c and the cathepsin G/alpha 1-proteinase inhibitor systems but not that for the alpha 1-proteinase inhibitor/pancreatic elastase or trypsin pairs.”

“In addition to the large number of known biological molecules which bind heparin, we show that heparin will form strong complexes, in solution, with thrombin (Kass ⋍ 109 l/mole); plasmin (Kass ⋍ 108 l/mole) and trypsin (Kass ⋍ 0.2 × 108 l/mole).”

“ Heparin , in low concentrations, can inhibit the clotting effect of trypsin by increasing plasma antithrombic activity.”

“ Heparin prevents fatal embolism which otherwise results in a high percentage of cases following the intravenous injection of treated trypsin in rabbits.”