Possible Interaction: Heparin and Oligosaccharides

“In addition, inhibition by oligosaccharides derived from depolymerized heparin increased with fragment size; partial inhibition was observed with oligosaccharides as small as hexamers.”

“In optical biosensor binding assays, competition by oligosaccharides for binding of HGF/SF to immobilized heparin showed that disaccharides failed to compete, whereas tetrasaccharides inhibited HGF/SF binding (IC50 8 μg/ml).”

“The resulting decreased conformational flexibility of the Asn-135 oligosaccharide and its close vicinity to the heparin binding site destabilize the activated relative to the native conformation.”

“The oligosaccharide at Asn-135 thus at most moderately interferes with the initial, weak binding of heparin to alpha-antithrombin.”

“However, low‐ and high‐grade SO in high concentrations (0·11 g/l) inhibited serotonin release induced by HAT sera and heparin.”

“Only high‐grade SO which inhibited heparin binding to platelets caused platelet activation with HAT sera.”

“Since the oligosaccharides formed have only 10 per cent of the anticoagulant activity of the heparins tested, it is conceivable that the platelet enzyme may play an important role in the inactivation of some of the biological properties of heparin.”