Possible Interaction: Heparin and Calcium Supplement

“ Heparin leads to a concentration-related reduction of calcium ions in blood.”

“The initial rate of thrombin (4.0 nM) inhibition by antithrombin III (200 nM) in the presence of heparin (2.5 ng/ml) decreased from 3.6 nM/min (in the absence of calcium) to 0.12 nM/min in the presence of 10 mM calcium.”

“ Heparin stimulated calcium sensitive gelatinolytic activities of approximately 22, 42, and 60kDa, but did not affect activities of MMP-2, MMP-9, or the 75kDa caseinolytic activity.”

“These antibodies or calcium selectively modulate interactions of fibronectin, heparin , sulfatide, alpha3beta1 integrin, tumor necrosis factor-alpha-stimulated gene-6 protein, and, to a lesser extent, alpha4beta1 integrin with native thrombospondin-1 but not with the truncated protein.”

“Binding of a full-length heparin chain to this site of factor Xa in the presence of calcium makes a significant contribution to acceleration of the proteinase inhibition by antithrombin through a ternary complex bridging or template mechanism.”

“ Heparin (200 UI/mL) decreased the free calcium concentration in Tyrode solution to 0.8 mM. Such a lowering of the free calcium concentration diminished vascular contractile reactions.”

“CONCLUSION Zinc he arin pr oduces a significant positive bias in the simultaneous determination of ionized magnesium and ion ized ca lcium concentrations.”

“However, the presence of calcium in EB heparin significantly increased measured total calcium concentrations, whereas the new CNLZ heparin did not interfere in total calcium determinations.”

“In a … mixture containing calcium ions and approximately equimolar concentrations of relipidated tissue factor (8.9 nmol/L) and factor VIIa (10 nmol/L), AT III (100 micrograms/mL) plus heparin (1 U/mL) inhibited 50% of the factor VIIa coagulant activity of the reaction mixture within 5 minutes.”

“More importantly, heparin abolished GTP gamma S (2.5 microM) induced Ca2+ release (IC50 10 micrograms/ml).”