Possible Interaction: Gossypol and Nitric Oxide

“All dosages of gossypol used increased the production of NO and all dosages except 200 μM raised the level of apoptosis.”

“Furthermore, pre-incubation with gossypol , an inhibitor of the formation and release of endothelium-derived relaxing factors (EDRF), completely abolished the effects of SP without influencing the dilatory action of CGRP.”

“The Ca2+ influx triggered by 3 and 10 µmol/L gossypol caused NO release and cell death, respectively.”

“ Gossypol , an inhibitor of EDRF , virtually abolished the 5-HT-induced relaxation while indometacin, an inhibitor of cyclooxygenase and accordingly of PGI2 formation, did not.”

“Incubation of unrubbed arteries with the irreversible inhibitor of EDRF, gossypol (10(-5) M), significantly increased the contractile response to 5-HT (Emax = 77 +/- 4%).”

“5 Gossypol (1–15 μm), a presumed inhibitor of EDRF biosynthesis, caused a marked drop in perfusion pressure followed by vasoconstriction.”

“To test whether inhibitors of endothelium-derived relaxation factor would potentiate the myogenic response of 3As, methylene blue or gossypol was topically applied to the cremaster muscle.”

“ Gossypol (10 microM, 5 min), an inhibitor of endothelium-derived relaxing factor (EDRF) production and/or release, increased basal RVR by 5% and significantly inhibited the vasodilator effects of ACh and ATP but had no effect on verapamil- or GTN-induced decreases in RVR.”

“Two inhibitors of EDRF , methylene blue and gossypol , increased RVR in nonischemic kidneys by 45 +/- 6 and 46 +/- 11%, respectively, an increase that was comparable to that found with ischemia alone (55 +/- 7%).”

“In vitro, gossypol is an inhibitor of the production and/or release of endothelium-derived relaxing factor.”