Possible Interaction: Glycine and Aspartate

“Substitution of glycine‐91 by site‐directed mutagenesis with either aspartate or alanine resulted in a significant decrease in transport activity of GLUT1 expressed in Xenopus oocytes.”

“Substitution of glycine , glutamine or serine attenuated the extent of block whereas the negatively charged aspartate enhanced it, consistent with the side chain of the native asparagine at this position contributing to a blocking site for intracellular Mg2+.”

“From these data we propose that the carboxyl group of this aspartate interacts with the amino moiety of glycine and the equivalent group contained in other agonists and antagonists.”

“We showed that the endogenous level of glycine (about 3 mM) was significantly higher than for other candidates such as L-glutamate, L-aspartate , or gamma-aminobutyric acid.”

“ Asp and Gly applied together at maximal concentrations (5 mM and 10 microM, respectively) evoked an inward current due to activation of N‐methyl‐D‐aspartate (NMDA) receptors.”

“Responses to glutamate and aspartate , which are endogenous neurotransmitter candidates, were enhanced by adding glycine.”

“Addition of glycine , arginine and cysteine to aspartate raised the hypocholesterolemic effect.”

“In silico point mutation studies showed that substitution of aspartate with glycine within a GTPase motif showed the largest decrease in stability and pH differentiation also affect protein's stability.”

“Mutational analysis on this motif shows the large decrease of stability after mutation of aspartate residue with glycine.”

“The N‐methyl‐D‐aspartate receptor (NMDAR) coagonist glycine potentiated D‐Asp currents only at −30 mV, while D‐serine did not potentiate D‐Asp currents at any amplitude.”