Possible Interaction: Glycerol and Trehalose

“When used in combination, the glycerol and trehalose combination provides the best tissue protection.”

“ Trehalose and its combinations with biostab, sucrose or glycerol significantly increased stability of HRP2 by 57% (p < 0.001).”

“ Glycerol anti-plasticizes the matrix with respect to the trehalose by enhancing the stability of the protein in a more rigid trehalose/water/glycerol matrix.”

“The analysis of the Amide I band reveals that glycerol enhances the stabilization effect of trehalose on proteins for low water content, but still liquid, systems.”

“We conclude that, in our SL-based extender, a combination of 100 mM trehalose and 5% glycerol was the most adequate combination to achieving post-thawing quality in our soybean lecithin-based extender, and our results support that a synergistic effect among trehalose and glycerol exists.”

“The synergic effect of trehalose and glycerol resulted in better cryosurvival of boar spermatozoa than that of a single cryoprotectant.”

“They inhibited the response to glycerol , specifically and competitively as compared with the almost total lack of effects of the reagent on the responses to the four sugars sucrose, D-glucose, D-fructose and trehalose.”

“These observations suggest that trehalose can be used in combination with glycerol as a cryoprotectant and that a high rate of viability can be achieved after a one-step dilution of the cryoprotectants.”

“For both, pure and crude glycerol, the highest amount of dry biomass leveled off at about 4 g/L. While the use of crude glycerol had no effect on the final concentration of biomass, it reduced the accumulation of trehalose in the cells.”

“By contrast, during the secondary drying stage, glycerol significantly enhanced the stabilizing effect of trehalose in the same sample, by replacing water-trehalose H-bonds with stronger glycerol-trehalose H-bonds and stiffening the amorphous trehalose matrix.”

“It was found that the addition of a small amount of glycerol had a plasticizing effect on the glassy trehalose-water matrix during the primary drying stage and then reduced the bioprotective effect of trehalose.”

“This opportunity was used for determining how a small amount of glycerol enhances the bioprotective efficiency of trehalose during FD of lysozyme formulations.”

“A detailed analysis of the hydrogen bonds (HBs) formed between species reveals that water and glycerol may antiplasticize the fast dynamics of lysozyme and trehalose by increasing the total number and/or the strength of the HBs they form in glassy matrices.”