Possible Interaction: Glycerol and Alpha-Chymotrypsin

“Kinetic and thermodynamic analysis using kinetic measurements of the thermal stability of alpha-chymotrypsin showed a higher inactivation rate in neat glycerol as compared to water in 30 to 45 degrees C range, however, when temperature increases enzyme stability in glycerol is better than water.”

“For example, at approximately 55 degrees C and 4.7 kbar, enzymatic activity of CT in the presence of 40% (v/v) glycerol is severalfold higher than in aqueous buffer.”

“The protection effect of glycerol on the catalytic activity and stability of CT becomes especially pronounced when both extreme factors of temperature and pressure reach critical values.”

“There has been observed an additivity in stabilization effects of high pressure and glycerol : thermal inactivation of pressure-stabilized CT can be decelerated in a supplementary manner by addition of 40% (v/v) glycerol.”