Possible Interaction: Glutathione and Trypsin

“When these GSH-dependent parameters were assayed during the coarse of the growth of an individual passage of cells, GSH levels declined following trypsinisation , but the activities of SeGSHpx and GSSGred remained essentially unaltered, when the data were standardised to cell number.”

“Subculture of the cells with trypsin decreased the cellular content of the major low-molecular-weight thiol, i.e., reduced glutathione , although the glutathione content had returned to levels comparable to those before subculture already after 4 h in conjunction with cell attachment.”

“Passage of cells with trypsin pertubs the cellular thiol homeostasis and causes a 50% decrease in the GSH content, whereas the total cysteine content is subsequently increased severalfold during cell attachment.”

“When the rebound of GSH following BSO exposure was reexamined, the GSH levels were found to rise rapidly following trypsinizing and plating.”

“The treatment of hemoglobin with a 40-fold molar excess of reduced glutathione or cysteine at 37 degrees C for 4 enhanced its susceptibility to trypsin 4 or 6 times, respectively.”

“ Glutathione has been shown to inhibit trypsin induced proteolytic activity.”

“Both GSH and TLCK prevented these effects of trypsin.”