Possible Interaction: Glutathione and Thioproline

“Our results show that OTZ increases blood glutathione levels, which may improve antioxidant status in dialysis patients.”

“Treatment of calcifying cells with OTC blocked the reduction in expression of both enzymes and prevented the decline in GSH.”

“Here we compare the ability of N‐acetylcysteine (NAC), 2‐oxothiazolidine‐4‐carboxylic acid (OTC), glutathione ethyl ester (GSH‐EE) and N‐acetylcysteine ethyl ester (NACET) to increase the intracellular concentration of GSH using primary human umbilical vein endothelial cells (HUVEC) as in vitro model.”

“The results presented in this report show that by augmenting the intracellular concentration of glutathione in mesothelial cells by the addition of OTZ to the dialysis fluid, we can increase their resistance to the acute toxicity of free radicals and long-term toxicity of glucose.”

“ OTC is known to enhance glutathione production in cells in which glutathione was depleted by reactive oxygen species.”

“GSH assays showed that treatment of U937 and Jurkat T-cells with NAC and OTC moderately increased the GSH level, while HC led to a significantly higher increase of the thiol level.”

“In contrast, there was a significant increase in the average concentration of cysteine from 0.37 to 0.99 nmol/mg of protein and of glutathione from 8.7 to 15.6 nmol/mg of protein in lymphocytes 2 to 3 hr after ingestion of OTC.”

“A reducing agent, L‐2‐oxothiazolidine‐4‐ carboxylic acid OTC ), a prodrug of cysteine, increases intracellular GSH.”

“CBS−/+ mice had significantly lower GPx‐1 activity compared with their CBS+/+ littermates, and OTC treatment led to a modest increase in tissue GPx‐1 activity and significant increases in total thiols and in reduced glutathione levels in both CBS+/+ and CBS−/+ mice.”

“Supplementation with N-acetylcysteine (NAC) and L-2-oxothiazolidine-4-carboxylic acid replenished both glutathione pools and provided protection from apoptosis.”