Possible Interaction: Glutathione and Sulforaphane

“Oxidative stress was reduced in animals undergoing epileptogenesis by a transient treatment with N-acetylcysteine and sulforaphane , which act to increase glutathione levels through complementary mechanisms.”

“The … SFN recorded a higher (p < 0.05) active nuclear Nrf2 protein (12.0 ± 0.4 vs …), mRNA Nrf2 (2.0 ± 0.3 vs 1.4 ± 0.1 fold increase), total GSH (384.0 ± 9.0 vs 256.0 ± 8.0 … apoptosis elicited by 6-OHDA compared to ERN.”

“sulforaphane SFN ) stimulates Nrf2 nuclear translocation and an increase of the reduced form of glutathione leading to resolution of lung inflammation but not AHR in Nrf2+/+.”

“Additionally, sulforaphane treatment inhibited the rotenone-induced reactive oxygen species production, malondialdehyde (MDA) accumulation, and resulted in an increased level of total glutathione and reduced glutathione (GSH): oxidized glutathione (GSSG) in the brain.”

“ SFN treatment also caused a rapid and significant depletion of GSH levels.”

“ SFN could activate the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) and increase the expression of the heme oxygenease-1 (HO-1) gene and production of glutathione.”

“ SFN stimulated the activity of the enzyme γ-glutamylcysteine ligase (γ-GCL), leading to increased synthesis of glutathione (GSH).”

“The activation of the nuclear factor E2-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway and the induction of intracellular glutathione GSH ) played an important role in the cytoprotective effects of SFN metabolites.”

“The inhibitory effects of SFN could be abolished by exogenously supplied GSH and by the GSH replenishing antioxidant N-acetylcysteine (NAC).”

“ Sulforaphane produced the most potent effect, increasing GSH by up to 2.4-fold.”