Possible Interaction: Glutathione and Phenobarbital

“However, 4 mM propylthiouracil did lead to a 50% increase in GSH reductase activity, and phenobarbital at 4 mM completely abolished GSH peroxidase and GSH reductase activity and led to a significant loss of viability.”

“RESULTS Chronic feeding of p-dimethyl … benzene (p-DAB) and phenobarbital (PB e levated the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma glutamyl transferase (GGT), … (LDH), triglyceride, cholesterol, creatinine and bilirubin and lowered the levels of glutathione (GSH ), glucose-6-phosphate dehydrogenase (G-6-…), catalase and HDL-cholesterol.”

“Only phenobarbital pretreatment also significantly reduced GSH and ATP depletion.”

“Inducers of microsomal enzymes aroclor 1254 and phenobarbital notably inhibited glutathione S-transferase activity in animals with experimental pancreatitis on days 2–4 of the disease (3.1–2.5 times in comparison with sham-operated induced animals).”

“Furthermore, we observe that the induction of chloramphenicol acetyltransferase (CAT) activity from an EpRE Ya-cat gene construct and of AP-1 binding activity by phenobarbital is inhibited by the thiol compounds N-acetyl-L-cysteine and glutathione.”

“… phenobarbital (80 mg/kg body weight, 4 days), ethanol (0.9 gm/kg body …), or diethylmaleate (3.9 mmol/kg body weight) resulted in changes in biliary glutathione secretion of +114%, -56%, and -95%, respectively, and in hepatic glutathione … -0%, +25%, and -86%, respectively, when compared with control values.”

“Pretreatment of rats with phenobarbital or beta-naphthoflavone induced GSH S-transferases but did not increase the in vivo formation of DNA adducts, suggesting that concomitant induction of cytochrome P450 might abolish the effect of induction of GSH S-transferase by increasing the oxidation of ethylene dibromide.”

“Our results clearly demonstrate that GSH S-transferase expression in seminal vesicles and pituitary glands can be suppressed by phenobarbital and diethylstilbestrol, respectively.”

“It is assumed that phenobarbital and PCN enhance the biliary excretion of these metals by increasing the transport of GSH , the carrier molecule, from liver to bile.”

“N-Benzylimidazole, an inhibitor of cytochrome P-450, completely blocked the response while phenobarbital pretreatment of the rats doubled the rate of GSH efflux.”