Possible Interaction: Glutathione and Phenethyl Isothiocyanate

“In vitro, PEITC decreased reduced glutathione (GSH), which resulted in decreased GSH/oxidized glutathione (GSSG) ratio and increased glutathionylation of Mcl-1, leading to rapid proteasomal degradation of Mcl-1.”

“In p53-defficient CLL cells, PEITC induced a rapid depletion of glutathione and a severe accumulation of ROS, leading to massive leukemia cell death in the stromal microenvironment.”

“These results indicate that GSH plays an important role in the MRP2-mediated transport of PEITC.”

“ PEITC lowered GSH levels, which was prevented by NAC, whereas ASC, TRX and DFO neither inhibited nor enhanced the GSH-lowering effect of PEITC.”

“Moreover, investigated results showed that PEITC inhibited the expression of superoxide dismutase (SOD) and glutathione (GSH), and caused oxidative stress to tumor cells.”

“ PEITC caused GSH redox stress in different ways in two cell types, because N-acetylcysteine (NAC) prevented redox stress in Chang but not in KKU-M214 cells.”

“The loss of mitochondrial transmembrane potential was induced by PEITC concurrent with GSH stress, but was not a primary cause of cell death.”

“ Phenethyl isothiocyanate (PEITC) is a naturally occurring electrophile which depletes intracellular glutathione ( GSH ) levels and triggers accumulation of reactive oxygen species (ROS).”

“Moreover, BITC and PEITC increased ROS generation and caused GSH depletion.”

“In contrast, benzyl isothiocyanate induced more thioredoxin nuclear accumulation (10 microM, 2.9-fold), increased production of ROS, and gave the greatest induction of thioredoxin reductase 1 mRNA (10 microM, 10.2-fold), whereas phenylethyl isothiocyanate was more potent in the depletion of reduced glutathione levels.”

“The cytotoxicity of phenethyl isothiocyanate was prevented by a high concentration of GSH (15 mM) and delayed by the antioxidant and c-Jun N-terminal kinase signalling pathway inhibitor curcumin.”