Possible Interaction: Glutathione and Nitroprusside

“The reduction of sodium nitroprusside by ascorbate and glutathione results in formation of the nitroprusside nitroxide radical which, on freezing at 77 K, results exclusively in the tetracyano [Fe(CN4)NO]2- and pentacyano [Fe(CN5)NO]3- forms of nitroxide radicals, respectively.”

“Thiol compounds such as glutathione , cysteine, and cysteamine reduce NP to generate NP nitroxide radical anion and a paramagnetic species characterized as a dithiolated dinitroso iron complex (DDIC), Fe(NO)2(RS)2 g = 2.030, A(14N) = 2.2 G(2N), and A(1H) = 1.1 G(4H).”

“ SNP (1.5 mM) was found to increase glutathione levels by 2-fold, as well as GCS activity by 6-fold.”

“In the reaction of reduced glutathione GSH ) with SNP , [(CN)5FeNO]3- is formed as well as low concentrations of an EPR-detectable GSH-SNP adduct.”

“In animals receiving MEL, the highest levels of GSH were observed in all the organs as compared to the control after 3, 6 h. Meanwhile, NP decreased G SH concentration in the blood, brain, liver and kidneys in all time intervals.”

“S-Nitrosoglutathione and sodium nitroprusside caused partial inhibition of DEA/NO-stimulated sGC that was prevented by GSH , whereas nitroglycerin (0.3 mM) had no effect.”

“Hyperglycaemia was accompanied by a significant increase in tissue TBARS and a decrease in NOx, GSH and anti‐oxidant enzymes, whereas, supplementation with l‐Arg and SNP significantly reduced TBARS levels and increased GSH and anti‐oxidant enzyme activities.”

“… GSH … SNP (0.1-0.5 mM), and this was preceded by the induction of mRNA for gamma-glutamylcysteine synthetase (gamma-GCS; the rate-limiting enzyme …, significantly suppressed the SNP-induced elevation of GSH protein and gamma-GCS mRNA, while curcumin, an inhibitor of AP-1, was less effective.”

“SNAP (an NO(+) generator) and SIN-1 (a peroxynitrite generator) both caused increases in SN56 GSH levels; in contrast, SNP caused an immediate and rapid decline in GSH.”

“ SNP spontaneously liberates nitric oxide in the presence of reducing substances like cysteine or glutathione , ubiquitous in many different tissues.”