Possible Interaction: Glutathione and Nimesulide

“In HUH-7 cells incubated for 24 h without albumin, nimesulide (1 mM) decreased the ΔΨm and cell NAD(P)H and increased the glutathione disulfide/reduced glutathione ratio and cell peroxides; nimesulide triggered MPT, ATP depletion, high cell calcium, and caused mostly necrosis, with rare apoptotic cells.”

“The protective effect of GSH , but not of BHA, was increased by nimesulide.”

“ Nimesulide exposure decreased GSH content (92.9%) and increased reactive oxygen species (9.29 fold) which was attenuated in Fp treated rats.”

“Our study showed that, nimesulide (100 microM) treatment resulted into rapid depletion of GSH (60%) in isolated rat liver mitochondria and significant Ca(2+) dependent MPT changed.”

“ NIM administration (8 mg/kg body wt) for 7 days caused significant depletion of the levels of SOD, CAT and reduced GSH , along with the increased levels of lipid peroxidation.”

“Compared with vehicle, nimesulide significantly (P<0.05) reduced hippocampal glutathione depletion and lipid peroxidation, as assessed by the levels of malondialdehyde (MDA), 4-hydroxy-alkenals (4-HDA) and lipid hydroperoxides levels, even when the treatment was delayed until 6 h after ischemia.”

“ Nimesulide also decreased the levels of NAD(P)H and glutathione in hepatocytes, but the extent of the effects was less pronounced in relation to the energetic parameters; in addition, these effects did not imply the peroxidation of membrane lipids.”