Possible Interaction: Glutathione and Methylene Blue

“In the absence of glucose, methylene blue at concentrations above 5 microM increased intracellular oxidant stress, as manifest by oxidation of dihydrofluorescein and cellular GSH.”

“4 S‐NO‐Glu induced‐increase in intracellular cyclic GMP synthesis, but not the increase in ouabain‐sensitive Rb‐uptake, was completely inhibited by methylene blue in HCCSMC.”

“ Methylene blue stimulates the oxidation of glutathione in red blood cells in vitro and in vivo.”

“Under aerobic conditions, methylene blue oxidizes glutathione 30 times faster than the spontaneous autooxidation of glutathione.”

“Specifically, CQ, MB and ART increased the levels of hepatic GSH by 108, 124 and 98 %, respectively, at day 6.”

“ Methylene blue treatment increases GSH levels and PBG-synthetase activity in erythrocytes while liver and kidney GSH and GSSG levels remain constant.”

“With the addition of GSH , the peak current of MB oxidation at about -0.27 V (IMB) increases, while the signal of Ag oxidation at about 0.1 V (IAg) decreases.”

“The vasorelaxant effect of GSH in endotheliumintact rings was inhibited by glibenclamide (3 μM), methylene blue (10 μM) and N-nitro-L-arginine methyl ester (10 μM), indicating the involvement of membrane K+ channels and NO-cGMP pathway.”

“Ascorbic acid, glutathione and Na-thioglycollate stimulated while methylene blue inhibited the growth and N2-ase activity.”