Possible Interaction: Glutathione and Leukotriene C4

“As such, it has a critical function in the metabolism of glutathione and in the conversion of the leukotriene LTC4 to LTD4.”

“Taxol, VP-16, vincristine, and vinblastine were also poor inhibitors of LTC4 transport but inhibition by these compounds was enhanced by GSH.”

“Incubation of gastrointestinal tissues in the presence of glutathione decreased the amounts of leukotrienes D4 and E4, while release of leukotriene C4 was simultaneously increased.”

“ LTC4 was undetectable in most experiments but it was found to accumulate when reduced glutathione (10 mM) was added.”

“Accordingly, stimulation of VSMC with LTC4 reduces intracellular levels of glutathione , but does not affect apoptosis.”

“Reduced form of glutathione significantly increased the synthesis of LTC4 , but showed no action on the syntheses of LTD4 or LTB4.”

“Leukotrienes D4, E4, B4, oxidized glutathione (GSSG), cysteine, and mercaptoethanol … to displace 3H-LTC4 at 1 microM concentrations, while GSH inhibited binding with a Ki of 47 nM. Differential centrifugation of … membrane preparation to remove mitochondria resulted in Ki values for LTC4 and GSH of 14 … 23 nM, respectively.”

“ GSH (10 mM) and SB (45 mM) are effective inhibitors of conversion of LTC4 into functionally important levels of LTD4 by the guinea pig trachea.”

“In addition, GSH appears to enhance LTC4 responsiveness by increasing synthesis of a contractile 5-lipoxygenase product(s), possibly LTC4.”

“ LTC4 production increased in … manner for GSH concentrations from 10(-8) to 10(-2) M. On the contrary, the 35S detected in the ether phase and the 35S-… from RP-HPLC decreased inversely with the concentration of unlabeled GSH added but did not with the addition of L-cysteine.”