Possible Interaction: Glutathione and Hemin

“The oxidation of ascorbic acid in the presence of haemin was stimulated by GSH , suggesting that ascorbic acid can alter the mechanism of H(2)O(2) generation observed with GSH and haemin alone.”

“One method, a multiwell assay based on the inhibition of the interaction of haemin with glutathione (GSH), is sensitive in the 10 microM range, takes c. 1 h and is suitable for either a high throughput screen or rapid assay during natural product isolation.”

“ Hemin orchestrated a defensive pathway involving the management of cellular non-protein thiols, via an increase in GSH levels and secretion of cysteine.”

“EDTA, N‐acetylcystein and glutathione prevented the toxicity of hemin and hemin/MAA.”

“It has been found that the electrochemical activity of glutathione was increased greatly at the glassy carbon electrodes modified with 5,10,15,20-tetraphenylporphine ruthenium(II) carbenyl (RuTPP), meso-tetraphenylporphine copper(II) complex (CuTTP) and hemin.”

“In conclusion, the presence of a high-affinity site for the hemin and the fact that glutathione at physiological concentrations increased the affinity of this site, suggest the involvement of glutathione transferase pi in the hemin transport.”

“It was found that the affinity of hemin for the cytoskeletal membrane proteins as well as for the membrane lipid core was decreased in the presence of glutathione.”

“The hemolytic effect of hemin on red cell was much reduced in the presence of glutathione.”

“Pretreatment with hemin or DMDC reduced neutrophil infiltration and TNF-α, IL-1β, and MDA formation, and increased the levels of GSH and mucus in the gastric tissue.”

“Pre-treatment with hemin reduced gastric damage and MDA formation and increased GSH concentration in the gastric mucosa.”

“The results outline the role of GSH at physiological concentration in preventing erythrocyte damage by haemin.”