Possible Interaction: Glutathione and Glutathione Disulfide

“Furthermore, translating these findings to intracellular environment, the conversion of GSH in GSSG can significantly alter the metallostasis.”

“Data were analysed by ANOVA, p < 0.05 RESULTS: The Km of ascorbylperoxide for … μM and Vmax was 38.4 ± 2.5 nmol/min/ U. The presence of GSSG in intravenous solution has … redox potential of glutathione , activation of caspase-3 (apoptosis marker) … induced by PN(+L) or ascorbylperoxide.”

“Furthermore, it was shown that GSSG , in the presence of GSH , interacts directly with MT.”

“LTC4 uptake was also markedly reduced by the competitive inhibitor,S-decyl-glutathione, as well as by the MRP1 substrates 17β-estradiol 17-β-(d-glucuronide), oxidized glutathione , and vincristine in the presence of reduced glutathione.”

“As the disulfide form (GSSG) is present only in minimal amounts with respect to the reduced form, a small GSH autoxidation during sample processing can give erroneously high GSSG level.”

“The addition of glutathione disulfide alone had no effect on microsomal lipid peroxidation, however, it prolonged the protection afforded by glutathione , particularly in assays containing Fe+2/NADPH.”

“Since glutathione disulfide and glutathione are a pivotal redox pair, the modulation of intracellular glutathione levels is shown to change the cytotoxicity of drugs dependent on the redox cycle, such as adriamycin and bleomycin, as well as the oxygen dependent drug neocarzinostatin.”

“Addition of GSSG to kidney cells inhibited metabolism of the GSH conjugate of paracetamol to the mercapturic acid derivative.”

“Glutathione GSH ) and the reduced/oxidized glutathione (GSH/GSSG) ratio are lowered in oxidative stress conditions and may lead to increased oxidative toxicity.”

“The GSH + GSSG level in both ages groups after surgery was lower than in the control.”