Possible Interaction: Glutathione and Diethyldithiocarbamate

“Catalase as well as the antioxidants N-acetylcysteine (NAC) and glutathione GSH ) partially inhibited the cytotoxic effect of DDC + HOCbl, whereas ascorbate, pyruvate, and tiron, a scavenger of superoxide anion, had no cytoprotective effect.”

“ GSH blocked almost completely the inhibition by 20 microM of MeDDC sulfoxide and greatly diminished the inhibition by 200 microM of MeDDC sulfine.”

“ Diethyldithiocarbamate decreases superoxide dismutase activity and, to a lesser extent, the total glutathione level.”

“The authors' previous work has indicated that the protective agent diethyldithiocarbamate (DDTC) prevents decrease in glutathione ( GSH ), alteration of antioxidant enzyme activity, and disruption of cochlear function with cisplatin administration.”

“The levels of glutathione and glutathione peroxidase were significantly decreased after treatment with ethanol, DDC and DEM, and this decrease was prevented by BTE pretreatment in the aforesaid manner.”

“ DDC was a less effective inducer of cystine uptake and increased intracellular GSH levels than was DSF.”

“Exogenous sulfhydryl compounds including GSH and cysteine partially blocked the induction of cystine transport by DSF or DDC , suggesting that the induction might be mediated through a sulfhydryl reaction between DSF and some cellular components.”

“ DDC produced a significant loss in liver reduced glutathione ( GSH ) level after 1 hr, but the nadir was observed later than that of Cu-SOD.”

“ GSH almost completely prevented the loss of microsomal enzyme activities induced by DTC and NADPH except for the drug metabolizing activities, in which protection was incomplete.”

“ DDTC also inhibits the effects of several structurally different tumor promoters and the greater GSH peroxidase and ODC responses produced by repeated TPA treatments.”

“The inhibitory effects of DDTC on TPA-decreased GSH peroxidase and TPA-induced ODC activities are additive with those of Na2SeO3 and D-alpha-tocopherol (vitamin E).”

“The interaction of DDC with reduced glutathione (GSH) was tested using … simple system based on the reduction of cytochrome c. When DDC (0.005 mM) … incubated with GSH (0.5 mM), the reduction of cytochrome c was eightfold greater … that expected from an additive effect of DDC and GSH.”