Possible Interaction: Glutathione and Buthionine Sulfoximine

“Suppression of intracellular GSH level by buthionine sulfoximine decreased DPD activity, while enhancement of intracellular GSH level by 2-mercaptoethanol increased DPD activity.”

“Pharmacological modulation of GSH level by BSO and NAC in DSePA treated cells led to abrogation and augmentation of cell kill respectively.”

“In vitro studies showed that arsenic exposure increased intracellular GSH content (SMD = 1.87, 95% CI (0.18, 3.56)) and promoted the expression …% CI (-2.46, -0.09)) and GCLC (SMD = −5.37, 95% CI (-5.37, -2.20)); siNrf2 inhibited the expression of GCLC, and BSO … GSH.”

“While the inhibition of the xCT-mediated uptake of cystine commonly causes ferroptosis, the sensitivity of cells to the inhibition of the glutathione (GSH) synthesis by buthionine sulfoximine (BSO) varies considerably.”

“GSH content in both lettuce and spinach were not significantly affected during depuration without BSO; whereas after treatment with BSO, GSH content significantly decreased by 36.0% and 24.7% in lettuce and spinach on 15 d, and the decrease remained on 18 d and 21 d in lettuce.”

“10 μM BSO treatment significantly depleted total GSH level in cancer cells, i.e. total GSH level decreased to one-fourth of the control in HCT116 cells while it decreased to two-fifth of the control treatment at SNU478 cell.”

“ GSH synthesis was inhibited with buthionine sulfoximine (BSO).”

“GSH quantification assays showed that camptothecin and BSO (an inhibitor of glutathione synthesis) strongly decreased intracellular GSH concentration in moDC, while infection with L. mexicana promastigotes had no effect in the level of GSH.”

“Buthionine sulfoximine BSO ) induces decreased glutathione ( GSH ) and trypanothione [T(SH)2] pools in trypanosomatids, presumably because only gamma‐glutamylcysteine synthetase (γECS) is blocked.”

“Indeed, recombinant γECS and TryS, but not GSH synthetase, were inhibited by BSO and kinetics and docking analyses on a TcTryS 3D model suggested BSO binding at the GSH site.”

“ BSO reduced GSH levels and promoted Cl2‐induced airway inflammation in wild‐type mice, but not in Nrf2‐deficient mice, whereas SFN suppressed Cl2‐induced airway inflammation in wild‐type but not in Nrf2‐deficient mice.”

“The inhibition of GSH synthesis by buthionine sulfoximine (BSO) decrease the anti‐oxidant defense leading to increased inflammation without affecting AHR. (”