Possible Interaction: Glutamine and Glycerol

“The use of glutamine at a higher concentration >100 mM in the presence of 2.5% of glycerol was toxic.”

“These experiments demonstrate that glutamine has a synergistic cryoprotective effect with glycerol on cryopreservation of stallion spermatozoa, and suggest that glutamine acts at the extra-cellular level, independently of glycerol.”

“In perfused kidney, although the increase in glucose output after addition of glutamine plus glycerol , hydrocortisone, or glucagon was similar in control and diabetic rats, glucose output after epinephrine infusion was significantly higher in diabetic rats than in control rats.”

“The data also demonstrate that there are complex age-related changes in the interaction of glycerol with 3-hydroxybutyrate and glutamine.”

“Acute renal failure induced by glycerol results in increased metabolism of glutamine by renal cortical slices of rats 16 and 36 hr after onset, and there is also increased glutamine uptake by the kidney in vivo.”

“PHA granule production was higher in filamentous than in rod-shaped cells, when glycerol (46.6 mM) was added to glutamine (5 mM) in co-metabolism, and fermentation was performed at a low-shaking speed.”

“However, alteration to aspartate, glutamate, and glutamine blocked passage of water, glycerol , and other organic solutes.”

“Quantification of free asparagine, aspartic acid, glutamine and glutamic acid hydrolyzed with hydrochloric acid or methanesulfonic acid in the presence of glycerol resulted in poor recoveries of aspartate and glutamate (between 6 and 66%).”

“Expression is also inhibited when glutamine is added to competence medium and when glycerol is substituted for glucose.”

“8.6% dimethyl sulfoxide plus 1.4% glycerol decreased Km for L-glutamine to 0.10 mM, while Km for MgATP was unaffected.”