Possible Interaction: Glucose and Methylene Blue

“ Methylene blue further enhanced reductase activity in mouse cells but only in the presence of glucose.”

“When 10−5M methylene blue was added with glucose , equivalent increases in rates of methemoglobin reduction occurred in the cells of all three species.”

“Further, although D-glucose enhanced reduction of methylene blue , it ameliorated the oxidant stress generated by the dye.”

“A protective effect of methylene blue with glucose on hemolysis was revealed.”

“In accordance with the role of NADPH, the hydroxylase activity of erythrocytes or hemolysates … dehydrogenase deficiency (i.e., with diminished capacity to form NADPH) displayed decreased responses to glucose or glucose … of methylene blue in comparison to samples from normal adults; maximal … addition of NADPH to the deficient hemolysates.”

“In 1928, Barron and Harrop (5) reported that in the presence of methylene blue , the disappearance of glucose from mammalian blood was accelerated.”

“From the studies presented here it is proposed that methylene blue activates a cyclic glucose oxidative pathway in mammalian red cells, and that this mechanism accounts for as much as 85 per cent of the oxygen consumed by human erythrocytes in the presence of methylene blue.”

“When islets from rats were incubated with 3 mg/ml of glucose, Methylene Blue (0.5, 1.0, 2.0 or 5.0 μg/ml) significantly decreased the concentration of NADPH, increased that of NADP+ and decreased the NADPH/NADP+ ratio in a dose-dependent manner.”

“Insulin release by glucose and L-Arg was also inhibited by methylene blue and LY83583 in islets.”

“Seven percent of D‐glucose flowed through the pentose phosphate pathway; this rate increased 11‐fold by methylene blue stimulation.”

“In contrast, the addition of glucose with methylene blue led to a 17-fold stimulation of the activity with both human and sheep erythrocytes.”