Possible Interaction: Genistein and Ng-Nitroarginine Methyl Ester

“Pretreatment with the nitric oxide synthase (NOS) inhibitor L-N-nitroarginine methyl ester (L-NAME, 100 μmol/L) significantly inhibited the effects of GST , high magnesium, and the combination of GST and magnesium.”

“These beneficial effects of genistein were blocked by a nonselective NOS inhibitor ( L-NAME ), but not by a selective iNOS inhibitor (aminoguanidine).”

“While a nitric oxide (NO) synthase (NOS) inhibitor, N(G)-nitro-l-arginine methyl ester (100 microM, 30 min) inhibited the relaxation, a PI3K/Akt inhibitor, LY294002 (10 microM, 30 min) or a tyrosine kinase inhibitor, genistein (30 microM, 30 min) was ineffective.”

“Both agents exhibited an antiaggregatory action, dependent on the nitric oxide release from vascular tissue, since preincubation of aortic strips with L-NAME partially and completely suppressed the inhibition of platelet aggregation induced by genistein or raloxifene respectively.”

“… genistein (… L-NAME , or by sodium orthovanadate (50 microg/kg), a potent inhibitor of protein tyrosine phosphatase; (2) genistein also decreased the PP of renal vascular … of mesenteric vascular bed in a dose-dependent manner, an effect which was partially inhibited by sodium orthovanadate, but unaffected by L-NAME.”

“The nitric-oxide synthase inhibitor N(omega)-nitro-L-arginine (100 microM) completely blocked the genistein , daidzein, and 17beta-estradiol-induced restoration of the relaxation response to carbachol, whereas the estrogen receptor antagonist ICI 182,780 (10 microM) had no effect on the relaxation responses.”

“Removal of the endothelium and pretreatment with L-NAME (100 microM) significantly inhibited relaxation at 3, 10 and 30 microM genistein and 10 and 30 microM daidzein.”

“ Genistein reduced circulating angiotensin-converting enzyme activity and angiotensin II concentrations in L-NAME rats.”