Possible Interaction: Gamma-Aminobutyric Acid and Tetrodotoxin

“ Basal GABA outflow in slices, but not in synaptosomes, appeared to be largely dependent upon neuronal activity, being prevented by tetrodotoxin (TTX).”

“Chronic suppression of activity by the application of tetrodotoxin (TTX) reduced mIPSC amplitudes and the levels of GAD67 and GABA.”

“ Tetrodotoxin reduced both pallidal and nigral GABA release without changing GLU levels.”

“ Tetrodotoxin inhibited both Ca2+-independent and Ca2+-dependent 4AP-evoked glutamate and GABA release equipotently, consistent with Na+ channel involvement.”

“ Tetrodotoxin (TTX) 6×10−7M, a concentration known to block neuronal mechanism but not to modify muscle membrane and anaphylactic histamine release, strongly prevented the inhibition caused by GABA in the Schultz-Dale reaction and in anaphylactic histamine release.”

“ Tetrodotoxin (TTX) (5×10−7mol/l) blocked the facilitation of spontaneous ACh release by GABA only when the slices were perfused with normal Krebs solution, but not when perfused with a KCl-enriched medium.”

“ TTX reduced basal monoamine and GABA releases without affecting glutamate release, whereas BFA did not affect them.”

“ Tetrodotoxin , which blocks sodium spiking in amacrine cells, and picrotoxin, which blocks the inhibitory action of GABA , only slightly reduced the surround.”

“In all cases, these responses were reduced by removing calcium from the saline and surangin B-evoked release of GLU, GABA , aspartic acid (ASP) and alanine was significantly inhibited by the sodium channel blocker tetrodotoxin.”

“In contrast, intra-mPfc TTX decreased local GABA (-33+6%) while VTA GABA levels were not affected.”