Possible Interaction: Fluoxetine and Calcium Supplement

“Collectively, this study shows that fluoxetine increased [ Ca 2 +] i in bladder cancer cells in a concentration-dependent fashion, by releasing Ca2+ from thapsigargin-sensitive Ca2+ stores in an IP3-independent manner, and by inducing Ca2+ influx from extracellular medium.”

“ Fluoxetine increased [ Ca 2 +] i concentration dependently (20-100 microM) with an EC50 value of 30 microM. The response was inhibited by 50-60% on extracellular Ca2+ removal.”

“Additionally, fluoxetine significantly suppresses endoplasmic reticulum (ER) calcium release and store-operated calcium entry (SOCE) activation, probably through reduction of ER calcium storage and inhibition of stromal interaction molecule 1 (STIM1) trafficking.”

“Taken together, we concluded that FLX exerts the inhibitory effect on electric field stimulation response in rat ileal smooth muscle by the inhibition of muscarinic receptors, decrease of intracellular calcium level by inhibiting phospholipase C and opens the potassium channels.”

“In all pretreated groups the calcium fluorescence was diminished by nifedipine 10(-7)M. Therefore, the pretreatment with amphetamine, fluoxetine or sibutramine seems to affect the calcium contractility and homeostasis in young rat vas deferens.”

“The results suggest that the alteration in the responsiveness of vasa deferentia from castrated rats to calcium could be responsible for increased sensitivity to the inhibitory effect of fluoxetine.”

“In addition, fluoxetine inhibited the HVA calcium current in carotid body type I chemoreceptor cells and pyramidal neurons prepared from prefrontal cortex.”

“ Fluoxetine inhibited Ca2+‐activated Cl− currents, ICl, Ca , activated by loading CPAE cells via the patch pipette with 1000 nM free Ca2+ (Ki=10.7±1.6 μM at pH 7.4, n=3‐5).”

“The inhibitory action of fluoxetine was not specific to K+ currents because it also inhibited both Ca2+ (IC50 = 13.4 microM) and Na+ (IC50 = 25.6 microM) currents in a concentration-dependent manner.”

“Increasing concentrations of Ca2+ (10−4–3×10−2 M) elicited arteriolar constrictions (up to ∼30%), which were markedly reduced by 2×10−6 M fluoxetine , whereas 10−5 M fluoxetine practically abolished these responses.”

“Extracellular 5-HT in the hippocampus was reduced by tetrodotoxin, 10 … increased by perfusion with a selective 5-HT reuptake inhibitor, fluoxetine , 10 microM. Addition of potassium (K+; 120 mM) to the … approximately 3-fold increase in 5-HT release, and a calcium free medium completely prevented …-HT release.”