Possible Interaction: Fluorides and NAD

“Exposure of red cells to fluoride produces a variety of metabolic alterations, most of which are based upon the secondary effects of enolase inhibition, which reduces pyruvate synthesis and interferes with the regeneration of diphosphopyridine nucleotide ( NAD).”

“ Fluoride competitively inhibits the catalytic activity of the enzyme and appears in a ternary enzyme-reduced nicotinamide adenine dinucleotide (NADH)-fluoride complex detectable in fluorescence and circular dichroism measurements.”

“ Nicotinamide adenine dinucleotide (NAD) apparently increased the degree of pyruvate reversal when fluoride , but not iodoacetate, was the inhibitory agent.”