Possible Interaction: Ethanol and Xylose

“It was shown that hap4Δ reveal 1.8-fold increase of ethanol production from xylose as compared to that of parental strain.”

“Moreover, whereas l‐arabinose was not consumed under oxygen limitation, d‐xylose was only slightly used, which resulted in low ethanol yield and productivity.”

“Through reduced overall metabolic flux, a more respiratory nature of consumption, and evading glucose signaling pathways, the bioconversion of xylose can be more amenable to redirecting flux away from ethanol towards the desired target product.”

“The evolved population also exhibited a better tolerance to ethanol when growing in a xylose medium supplemented with 35.5 g/L ethanol.”

“In batch fermentations using various concentrations of xylose (50-104g/L), higher xylose concentration enhanced xylose consumption rate and ethanol productivity but reduced ethanol yield, owing to the accumulation of xylitol and glycerol from xylose.”

“The higher consumption of xylose led to final ethanol titres that were approximately 15% higher.”

“Interestingly, some genes involved in glycolysis and ethanol fermentation were also repressed during growth on xylose , suggesting that these genes are good targets for engineering H. polymorpha to improve xylose fermentation.”

“ Ethanol production was always higher in a mixture of glucose and xylose than in glucose alone.”

“Increased initial cell density in the fermentation (from 5 to 20 g/L) did not increase the ethanol yield, but improved and accelerated xylose consumption in both cases.”

“However, efficiency of xylose utilization and ethanol yield was low.”