Possible Interaction: Ethanol and Phosphatidic Acid

“Furthermore, in carbachol‐stimulated cells, ethanol increased the formation of phosphatidylethanol and inhibited the formation of PA.”

“ Ethanol inhibited the accumulation of endogenous PA and calcium mobilization, indicating that activation of membrane phospholipase D plays a role in PA‐mediated Ca2+ influx.”

“ Ethanol , which competitively lowers phosphatidic acid ( PA ) production, caused dose‐dependent inhibition of superoxide (O2 ‐) generation after hyphal stimulation but altered neither baseline‐unstimulated O2 ‐ production nor responses to phorbol myristate acetate.”

“In cells labeled with L-lyso-3-PC 1-[1- 14C]palmitoyl, PLD but not IL-4, increased the production of phosphatidic acid (PA) and phosphatidyl-ethanol when pretreated with ethanol.”

“Inhibition of phosphatidic acid formation by wortmannin or ethanol reduced the microcrystal-stimulated production of superoxide anions.”

“In both cases, there was a concomitant rise of diacylglycerol (DG), and the PA accumulation was suppressed by ethanol at the expense of labeling of PEt.”

“As primary alcohols such as ethanol and 1-butanol were shown to inhibit the phospholipase D (PLD)-mediated formation of PA , a mitogenic lipid messenger, we tested their effects on ceramide formation.”

“ Ethanol reduces the PA :ceramide ratio in fetal astrocytes, a mechanism which likely participates in ethanol-induced glial apoptosis during brain development.”

“Concomitantly, ethanol caused the formation of phosphatidylethanol (PEth) by phospholipase D (PLD) and reduced PLD‐mediated formation of phosphatidic acid (PA).”

“Since the involvement of PLD activation in the early stages of pancreatic regeneration was postulated, our results suggest that chronic ethanol feeding can influence this process by decrease of PA production, probably because of the inhibition of hydrolytic PLD activity in the presence of ethanol.”

“J. 285, 395-400), the presence of ethanol decreases the α-thrombin-induced production of phosphatidic acid without affecting the induced increase in nuclear diglyceride, indicating that the increase in nuclear PLD activity is responsible for the effect on phosphatidic acid, but not that on diglyceride.”