Possible Interaction: Ethanol and Nitroarginine

“Cerebellar surface application of nitric oxide synthesis (NOS) inhibitor, L-NNA (5 mM) significantly decreased the amplitude of P1 and the pause of SS firing in EtOH exposure group, but did no effect on control group.”

“The NO donors and L-arginine applied as pretreatment prior to ethanol resulted in diminishing of its damaging action that was similar for all these drugs, while L-NNA intensified both the injury and the drop in the PD values caused by ethanol.”

“It is possible that this effect is not caused by the interaction of ethanol with nitric oxide pathways but by synergistic CNS depression caused by ethanol and L-NOARG.”

“ L-NOARG at doses of 20 and 40 mg/kg reduced the exploratory activity of rats in the open-field test and significantly enhanced the sedative effect of ethanol in this test.”

“Moreover, the combined administration of ethanol (2 g/kg) and L-NOARG (20 and 40 mg/kg) caused a decrease in the body weight of animals, observed for 14 days.”

“ L-NOARG also significantly increased the toxicity of ethanol as evidenced by increased post-experimental lethality.”

“Moreover, the nitric oxide synthase inhibitor L-nitroarginine blocks tolerance to the effects of ethanol.”

“Pretreatment with L-NNA decreased both mucosal blood flow and ISO2 in the basal period and enhanced decreases in both mucosal blood flow (2.7-fold) and ISO2 (4.3-fold) induced by 30% ethanol compared with controls.”

“RESULTS Pretreatment with L- NA si gnificantly increased macroscopic (7.7-fold) and microscopic damage caused by 30% eth anol. ”

“The first demonstrated that L-NA prevented the development of rapid tolerance to ethanol when injected prior to ethanol either on both Days 1 and 2 or only on Day 1.”

“We recently reported that the nitric oxide (NO) synthase inhibitor L-nitroarginine (L-NA) blocks the development of rapid tolerance to the motor incoordinating effect of ethanol in the tilt-plane test.”

“ Ethanol also inhibited the acetylcholine-induced relaxation resistant to nitro-L-arginine.”

“However, ethanol attenuated the reflex HR responses of NG-nitro-L-arginine and angiotensin II but not that of noradrenaline.”

“The results demonstrate that ethanol selectively but non-competitively inhibits the MAP response to NG-nitro-L-arginine , suggesting an interaction between ethanol and the L-arginine/nitric oxide pathway.”

“infusions of ethanol dose dependently reduced maximum MAP response to NG-nitro-L-arginine , with a Ki of 96 +/- 8 mg/kg per min but did not alter the ED50.”

“The cardiovascular effects of EtOH were significantly attenuated by ICV or by RVLM post treatment with ketamine (an NMDA receptor antagonist), N5-(nitroamidino)-L-2,5-diaminopentanoic acid ( L-NNA ; a NO synthase inhibitor), or bicuculline (a GABA receptor antagonist).”