Possible Interaction: Ethanol and Interleukin-10

“In contrast, the induction of IL-10 by LPS is enhanced by ethanol treatment, suggesting that elevated IL-10 may play a role in ethanol-induced suppression of IL-12.”

“ IL-10 increased ethanol induced apoptosis in primary culture rat hepatocytes (28%).”

“ Acute alcohol inhibited TLR8- or TLR4-induced TNF alpha protein and mRNA induction while it augmented IL-10 production in monocytes.”

“The mechanisms of IL‐10 induction by alcohol remain poorly understood.”

“Using specific Src (PP2), JNK1/2 (… p38 (SB203580) inhibitors, we determined that alcohol treatment alone induced and together with LPS, augmented the DNA‐binding capacity of … protein‐1 (Sp‐1) and AP‐1 transcription factors involved in IL‐10 production via Src‐mediated activation … p38 MAPK and JNK, respectively.”

“Reduced allostimulatory capacity of DCs treated with alcohol in vitro correlated with decreased co-stimulatory molecule (B7.1 and B7.2) expression, as well as with reduced interleukin (IL)-12 and increased IL-10 concentrations, in mixed lymphocyte cultures.”

“Experiments with anti-IL-10 neutralizing antibody show that ethanol may prevent monocyte IL-12 induction via IL-10.”

“Furthermore, ethanol significantly augmented LPS-induced monocyte IL-10 secretion at 18 h. Data also show that ethanol-induced changes in monocyte IL-10 mRNA levels mirror those seen at the protein levels.”

“Here, we tested the hypothesis that some of the regulatory effects of ethanol on monocyte functions are mediated by elevated M phi IL-10 production.”

“Physiologically relevant in vitro doses of ethanol (25-100 mM) resulted in significantly increased IL-10 secretion by normal blood monocytes after 18 h stimulation.”

“In contrast, acute ethanol treatment induces monocyte production of mediators with immunoinhibitory potential, including transforming growth factor-beta and IL-10.”

“We further show that ethanol not only induces monocyte/macrophage (Mø) IL-10 and transforming growth factor-beta, but even augments bacterial (both LPS and SEB) stimulation-induced production of both of these cytokines.”

“ Ethanol consumption decreased plasma levels of IL-10 in WT mice.”

“These findings provided novel evidence that IL-10 counteracted the initial mechanisms whereby ethanol induces its cardiorenal damages.”

“We hypothesized that IL-10 would limit the deleterious effects induced by ethanol consumption in the cardiorenal system.”

“Immunohistochemistry analyses determined that ethanol decreased IL-10 by 50 % in the basolateral amygdala (BLA) but had no effect on IL-4.”