“MD simulations of 200 mM GAG ensembles in pure water and in the aqueous solution with 42% ethanol showcase the ability of CHARMM36m to … average pPII content of alanine in GAG and provide a plausible explanation for this effect, which may stem from an increased propensity of GAG … ethanol.”
“Spectroscopic data, including three J-coupling constants and amide I' profiles, indicate that addition of 42% ethanol to water significantly reduces the pPII content of alanine residue in GAG.”