Possible Interaction: Ethanol and Fomepizole

“In cultured human or mouse HSC, production of CTGF, α-SMA and/or collagen was increased by ethanol treatment, an effect mimicked by acetaldehyde and blocked by 4-methylpyrazole (4-MP) or N-acetylcysteine (NAC).”

“Inhibition of EtOH metabolism by 4-methylpyrazole abolishes almost completely the Mg(2+) extrusion induced by the first dose of EtOH, and partially enlarges that elicited by the second dose of alcohol or the subsequent adrenergic stimulation.”

“ 4-Methylpyrazole , a specific inhibitor of the alcohol-metabolizing enzymes, abolished the effects of ethanol on hepcidin.”

“This inhibitory effect on the proteasome was blocked when ethanol metabolism was blocked by 4-methyl pyrazole.”

“Whereas inhibition of ethanol oxidation by 4-methylpyrazole blocked the effect, the aldehyde dehydrogenase inhibitor cyanamide enhanced the effect of ethanol in the hepatoma cells, supporting the idea that the effect is likely mediated by acetaldehyde.”

“The ADH inhibitor 4-methyl pyrazole (4-MP) reduced ethanol oxidation in the skin and liver in a concentration dependent manner: activity was reduced to approximately 30-40% and approximately 2-10% of the control activity, in the skin and liver, respectively, using 1 mM 4-MP.”

“Although 4-methylpyrazole is a potent inhibitor of some of the liver alcohol dehydrogenases, it is not very effective against all of the human isoenzymes, and it is a competitive inhibitor against alcohol , which makes it less effective when the concentration of substrate alcohol is increased.”

“Generation of the factor is specific for hepatocytes and is blocked by inhibiting ethanol metabolism with 4-methylpyrazole.”

“Chronic administration of ethanol and 4-methylpyrazole indicated that there is a mutual interaction in the metabolism of ethanol and 4-methylpyrazole , leading to a higher concentration of both ethanol and 4-methylpyrazole in the blood.”

“Combined treatment of ethanol and 4-methylpyrazole caused an increase of the microsomal drug-metabolizing activity.”

“Several clinical chemical parameters showed no impairment of liver or kidney function, except for an enhancement of the microsomal drug-metabolizing activity after concurrent administration of 4-methylpyrazole and ethanol.”