Possible Interaction: Ethanol and Asparagine

“However, the inhibitory effects of ethanol were reduced by expression of an NR1 C0 domain deletion mutant (NR1Δ839–863), truncation mutant (NR1858stop), or a triple-point mutant (Arg to Ala, Lys to Ala, and Asn to Ala at 859–861) previously shown to significantly reduce calcium-dependent inactivation.”

“Large amounts of additives (α - tocopherol, 0.5; N,…'-dipheny 1- p -phenylenediamine (DPPD), 1.5; asparagine , 10 mg per cal), reported by others to protect against steatosis following a single large … of ethanol were found to be ineffective in preventing the fatty liver produced by prolonged ethanol intake.”

“ Asparagine enhanced the rate of lactate-stimulated ethanol oxidation, particularly during the early part of the incubation.”

“Our studies suggest that asparagine or a low concentration of ammonia, by providing components of the malate/aspartate shuttle, can ameliorate some of the metabolic effects of ethanol on the liver.”

“The presence of asparagine also rapidly lowered the [lactate]/[pyruvate] ratio of hepatocytes incubated with lactate plus ethanol establishing a steady-state level of 15:1 within 10-15 min.”

“In hepatocytes from fed rats alanine, asparagine , cysteine, glycine, hydroxyproline, ornithine and serine still increase ethanol oxidation, although to a lesser extent than in cells from starved rats.”

“In hepatocytes from starved rats, asparagine , serine, ornithine, hydroxyproline, histidine, cysteine, alanine, glycine, glutamate, glutamine, aspartate and arginine significantly increase ethanol consumption.”

“In hepatocytes from fasted rats, alanine, arginine, asparagine , aspartate, citrulline, cysteine, glutamate, glutamine, glycine, histidine, hydroxyproline, ornithine and serine increase significantly ethanol consumption.”

“The … that L-asparagine prevents the decrease in hepatic ATP produced by each of these agents and protects against the fatty infiltration of the liver that … the administration of ethionine or small doses of CCl 4 , but not against that produced by large doses of CCl 4 or ethanol.”