Possible Interaction: Ethanol and Arachidonic Acid

“Arachidonic acid induced ERK1/2 phosphorylation and nuclear translocation of early growth response-1 (Egr1), and ethanol synergized with arachidonic acid to promote this effect.”

“These results indicate that ethanol acutely inhibits muscarinic receptor signaling through the arachidonic acid pathway in a noncompetitive manner, but chronically enhances muscarinic signaling through the same pathway.”

“ Arachidonic acid evoked irreversible platelet aggregation in all four preparations; this was significantly inhibited by the high dose ethanol in human and rat PRP, but not whole blood.”

“ Alcohol 4 …) arachidonic acid induced platelet aggregation of human platelet-rich plasma (I50 = 0.65 +/- 0.1 microM); (b) 11,9-epoxymethano-PGH2 induced contraction of guinea … rat aorta (pA2 = 8.1 +/- 0.2); and (c) arachidonic acid induced bronchoconstriction in the anesthetized guinea pig (1 mg/kg iv).”

“In other animal models, alcohol has been reported to alter blood platelet metabolism of arachidonic acid , to suppress the enzymatic degradation of PGs, and to alter the response of the adenyl cyclase system to several hormones including PGs of the "E" series.”

“This chapter reviews evidence for both direct and indirect biochemical interactions between ethanol and the metabolism of arachidonic acid and several related compounds.”

“Previous research has demonstrated that prolonged exposure to ethanol results in alterations in the oxidative metabolism of arachidonic acid including lowered membrane levels of arachidonate, increased arachidonate peroxidation, and increased oxidation of arachidonate to prostaglandin E2.”

“ Arachidonic acid altered cell proliferation and was cytotoxic mostly to cells engineered to overexpress CYP2E1 but both effects were significantly lower in cells pretreated with ethanol or acetate.”

“In cells pretreated with acetate or ethanol , the oxidative stress induced by arachidonic acid was also significantly lower.”

“Treatment with 2.0% (v/v) ethanol , an inhibitor of phospholipase D, decreased sulfur mustard-induced arachidonic acid release 40+/-7%.”

“This observation is consistent with the view that alcohol reduces platelet sensitivity to thrombotic stimuli by inhibition of arachidonic acid release and, therefore, subsequent thromboxane synthesis.”

“Conversion of [1-14C] palmitic to oleic acid and eicosatrienoic to arachidonic acid were inhibited in 400 mM ethanol treated cells suggesting an inhibition of delta 9 and delta 5 desaturase activity.”